The following page describes the simple process of using Pocket Diagnostic® lateral flow plant disease tests for the in-field testing of Phytophthora, Erwinia amylovora, Potato virus Y and Ralstonia solanacearum.
Always read the instructions provided with the kits before use.
Select symptomatic plant material from where the healthy material meets the diseased material. As a general rule, we recommend that the sample should weigh approx. 0.2g or 25x25mm in size. It’s important to wash any soil and other debris from the plant material sample prior to adding to the bottle.
Selection of the most appropriate sample ensures accurate and reliable results.
The unique ‘bottle and ball’ sample preparation method sufficiently breaks down plant material after only 30-60 seconds of shaking. After shaking the sample should be left to settle prior to a few drops being removed from the top of buffer and added to the tests device.
This easy and clean approach enables Pocket Diagnostic® plant disease tests to be used on the following plant material:
Hard materials may require slightly longer shaking to ensure adequate sample breakdown.
One line present only at the C line indicates a negative result.
Two lines present, at both the T and C line, indicates a positive result.
The absence of a line at C indicates the test has failed and must be repeated.
Below is an example of what can happen if too much debris and plant material are added to the test strip. There is too much debris preventing the test from working properly.
Always read the instruction before using Pocket Diagnostic®.
— Pascal Frey (@pascal_frey) 28 September 2016
— CHTS Ltd (@CHTSLtd) 5 August 2016
© Abingdon Health 2017