Pocket Diagnostic® plant disease rapid tests can be used to test wood samples. However, to make the most of the test’s sensitivity some simple steps on sample preparation should be followed
The key to success lies in ensuring the plant material to be tested is adequately disrupted to allow extraction of the pathogen into the buffer. This general guidance applies to all plant samples (leaves, stems and roots) which are hard or woody.
How to test wood samples
- Select a sample using a fine blade. Remove the outer bark or external root covering to reveal the soft bark. Samples need to include both the leading edge* and a small area just in front of this (*the area where the diseased part of the plant meets the healthy part of the plant). Note: the test does not work directly on ooze from the cankers.
- Samples must be broken down prior to placing in the extraction bottle. This is best done by finely slicing and crushing.
- Add the sample to the extraction buffer and secure the lid tightly.
- Shake the bottle vigorously for 60 seconds.
- Allow the plant debris to settle to the bottom and then draw up the extraction liquid into the pipette. Add 2-3 drops into the sample well avoiding adding any air bubbles or debris.
- Valid results appear within 10 minutes. Do not count any results after 10 minutes. One line is a negative result and two lines is a positive result.
For information about using Pocket Diagnostic® plant disease rapid tests to test wood samples please contact us on +44 (0) 1904 406050 or firstname.lastname@example.org.
Always read the instructions before using Pocket Diagnostic® rapid tests.