Tel: +44 (0) 1904 406050

PCRD Nucleic Acid Detector* is a rapid lateral flow immunoassay designed to detect and make visible the successful amplification of target DNA sequences in less than 10 minutes.

PCRD is a rapid, safe, and cost-effective alternative to using agarose gel electrophoresis and can be used in conjunction with the following amplification methods**:

  • Polymerase Chain Reaction (PCR)
  • Loop mediated isothermal amplification (LAMP)
  • Recombinase Polymerase Amplification (RPA)
  • Helicase dependent amplification (HDA).

How do customers use PCRD?

PCRD is used by customers globally from many different industry sectors. Read Abingdon Health’s article about rapid nucleic acid detection to learn more.

Advantages of PCRD

  • Safe to use – avoids intercalating dye and UV light
  • Compatible with different amplification methods
  • No special chemistry or equipment required
  • Rapid result – 10 minutes
  • High sensitivity
  • Multiple sample analysis in parallel
  • Cost effective

Interpretation

Qualitative visual detection of labelled amplicons using PCRD is easy to interpret. The test strips incorporate reagents which sequentially capture and allow the visualisation of double-stranded amplification products containing selected binding partners. The result is detectable by the naked eye due to an aggregation of carbon particles at the capture line.

PCRD nucleic acid detection LFDLine 1: Closest to the sample well, detects DIG/Biotin-labelled amplicons

Line 2: Detects FAM/Biotin – or FITC/Biotin-labelled amplicons

Line C: Furthest away from the sample well, is a flow-check control line

A single line visible at the C-line position indicates a negative result (no labelled amplicon present), while a visible line at Line 1 or Line 2, or both simultaneously, represents a positive result where amplicon has been detected.

While the test is not quantitative there is a correlation between line intensity and the amount of amplicon present.

How to order PCRD?

Online orders can be placed by visiting our online shop. Phone orders can be placed by calling +44 (0) 1904 406080, and to order on account or via proforma invoice please email info@abingdonhealth.com.

Further details about the nucleic acid detection assay can be found at abingdonhealth.com/nucleic-acid-detection-pcrd.

*PCRD Nucleic Acid Detector is manufactured and sold by Abingdon Health.
**Please note that protocols may differ between each method.