PCRD Nucleic Acid Detector is a rapid lateral flow immunoassay designed to detect and make visible the successful amplification of target DNA sequences in less than 10 minutes.
PCRD is a rapid, safe, and cost-effective alternative to using agarose gel electrophoresis and can be used in conjunction with the following amplification methods*:
- Polymerase Chain Reaction (PCR)
- Loop mediated isothermal amplification (LAMP)
- Recombinase Polymerase Amplification (RPA)
- Helicase dependent amplification (HDA).
*Please note that protocols may differ between each method.
PCRD is designed to detect amplicons labelled with DIG/Biotin and/or FITC (or FAM)/Biotin, therefore primers used in the amplification process must incorporate one or both of the ligand pairs. PCRD will only detect double stranded amplicons containing the ligand pairs, single-stranded DNA will not be detected.
PCRD nucleic acid detector offers a versatile and quick solution for confirming the presence of target DNA sequences following amplification and can be used in a number of applications. Examples include:
- Meat speciation testing
- Food and drink authenticity
- Rapid disease detection (veterinary, human and plant)
Advantages of PCRD
- Safe to use – avoids intercalating dye and UV light
- Compatible with different amplification methods
- No special chemistry or equipment required
- Rapid result – 10 minutes
- High sensitivity
- Multiple sample analysis in parallel
- Cost effective
Qualitative visual detection of labelled amplicons using PCRD is easy to interpret. The test strips incorporate reagents which sequentially capture and allow the visualisation of double-stranded amplification products containing selected binding partners. The result is detectable by the naked eye due to an aggregation of carbon particles at the capture line.
Line 1: Closest to the sample well, detects DIG/Biotin-labelled amplicons
Line 2: Detects FAM/Biotin – or FITC/Biotin-labelled amplicons
Line C: Furthest away from the sample well, is a flow-check control line
A single line visible at the C-line position indicates a negative result (no labelled amplicon present), while a visible line at Line 1 or Line 2, or both simultaneously, represents a positive result where amplicon has been detected.
While the test is not quantitative there is a correlation between line intensity and the amount of amplicon present.
PCRD Nucleic Acid Detector Assay Instructions For Use
For further information on our PCRD Nucleic Acid Detector and/or design and supply of labelled primers please contact the team on 01904 406080 or firstname.lastname@example.org.