Tel: +44 (0) 1904 406050

PCRD Nucleic Acid Detector is a rapid lateral flow immunoassay designed to detect and make visible the successful amplification of target DNA sequences in less than 10 minutes.

PCRD is a rapid, safe, and cost-effective alternative to using agarose gel electrophoresis and can be used in conjunction with the following amplification methods*:

  • Polymerase Chain Reaction (PCR)
  • Loop mediated isothermal amplification (LAMP)
  • Recombinase Polymerase Amplification (RPA)
  • Helicase dependent amplification (HDA).

*Please note that protocols may differ between each method.

PCRD is designed to detect amplicons labelled with DIG/Biotin and/or FITC (or FAM)/Biotin, therefore primers used in the amplification process must incorporate one or both of the ligand pairs. PCRD will only detect double stranded amplicons containing the ligand pairs, single-stranded DNA will not be detected.

Applications

PCRD nucleic acid detector offers a versatile and quick solution for confirming the presence of target DNA sequences following amplification and can be used in a number of applications. Examples include:

  • Meat speciation testing
  • Food and drink authenticity
  • Rapid disease detection (veterinary, human and plant)

Advantages of PCRD

  • Safe to use – avoids intercalating dye and UV light
  • Compatible with different amplification methods
  • No special chemistry or equipment required
  • Rapid result – 10 minutes
  • High sensitivity
  • Multiple sample analysis in parallel
  • Cost effective

Interpretation

Qualitative visual detection of labelled amplicons using PCRD is easy to interpret. The test strips incorporate reagents which sequentially capture and allow the visualisation of double-stranded amplification products containing selected binding partners. The result is detectable by the naked eye due to an aggregation of carbon particles at the capture line.

PCRD nucleic acid detection LFDLine 1: Closest to the sample well, detects DIG/Biotin-labelled amplicons

Line 2: Detects FAM/Biotin – or FITC/Biotin-labelled amplicons

Line C: Furthest away from the sample well, is a flow-check control line

A single line visible at the C-line position indicates a negative result (no labelled amplicon present), while a visible line at Line 1 or Line 2, or both simultaneously, represents a positive result where amplicon has been detected.

While the test is not quantitative there is a correlation between line intensity and the amount of amplicon present.

PCRD Nucleic Acid Detector Assay Instructions For Use

 

Contact Us

For further information on our PCRD Nucleic Acid Detector and/or design and supply of labelled primers please contact the team on 01904 406080 or info@abingdonhealth.com.